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Abstract for MAO-Glo™ Assay
The MAO-Glo™ Assay provides a homogeneous luminescent method for
measuring monoamine oxidase (MAO) activity from recombinant and native
sources and measuring the effects of test compounds on MAO activities.
The MAO-Glo™ Assay is performed by incubating the MAO enzyme with
a luminogenic MAO substrate. The substrate of the MAO-Glo™ Assay
is a derivative of beetle luciferin [(4S)-4,5-dihydro-2-(6-hydroxybenzothiazolyl)-4-
thiazolecarboxylic acid]. MAO converts this luciferin derivative to methyl
ester luciferin. After the MAO reaction has been performed, the reconstituted
Luciferin Detection Reagent is added to simultaneously stop the MAO reaction,
convert the methyl ester derivative to luciferin and produce light.
This addition initiates a stable glow-type luminescent signal with a half-life
greater than 5 hours. The amount of light produced is directly proportional
to the activity of MAO.
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