Abstract for HisLink™ 96 Protein Purification System

Part# TB342
Printed in USA. Revised 6/09.
Instructions for Use of Products V3680 & V3681.

The HisLink™ 96 Protein Purification System provides a simple, quick and robust method of purifying multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum-based method. The system is designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates. The HisLink™ System is amenable to manual or automated methods, such as the Beckman Coulter Biomek^® 2000 or FX for high-throughput applications.

In preparation for protein purification, bacterial cells expressing a polyhistidine- or HQ-tagged protein are lysed directly in culture using the provided FastBreak™ Cell Lysis Reagent. The HisLink™ resin is added directly to the lysate, mixed, and the polyhistidine- or HQ-tagged proteins bind in a matter of minutes.