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Abstract for HisLink™ 96 Protein Purification System
The HisLink™ 96 Protein Purification System provides a simple,
quick and robust method of purifying multiple polyhistidine- or HQ-tagged
expressed proteins from
E. coli using a vacuum-based method.
The system is designed to purify expressed polyhistidine- or HQ-tagged
proteins directly from deep-well, 96-well plates. The HisLink™
System is amenable to manual or automated methods, such as the
Beckman Coulter Biomek® 2000 or FX for high-throughput
applications.
In preparation for protein purification, bacterial cells expressing
a polyhistidine- or HQ-tagged protein are lysed directly in culture using
the provided FastBreak™ Cell Lysis Reagent. The HisLink™ resin is
added directly to the lysate, mixed, and the polyhistidine- or HQ-tagged
proteins bind in a matter of minutes.
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