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Abstract for Pgp-Glo™ Assay Systems
The Pgp-Glo™ Assay Systems provide the necessary reagents
for performing luminescent P-glycoprotein (Pgp) ATPase assays. The
Pgp-Glo™Assay detects the effects of compounds on recombinant human Pgp in a cell membrane fraction. The assay relies on the ATP
dependence of the light-generating reaction of firefly luciferase.
ATP is first incubated with Pgp, then the Pgp ATPase reaction is
stopped, and the remaining unmetabolized ATP is detected as a
luciferase-generated luminescent signal.
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