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Abstract for MagZ™ Protein Purification System
Purification of a polyhistidine- or HQ-tagged protein that has been expressed in rabbit reticulocyte lysate is currently limited by hemoglobin in the lysate copurifying with the protein of interest. Hemoglobin copurification limits downstream applications (e.g., fluorescence-based functional assays, protein-protein interaction studies) and reduces the amount of protein purified. Thus, we developed a unique system that purifies
polyhistidine- or HQ-tagged proteins expressed in rabbit reticulocyte lysate. These
polyhistidine- or HQ-tagged proteins are 99% free of the contaminating hemoglobin.
The MagZ™ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed
polyhistidine- or HQ-tagged protein from rabbit reticulocyte lysate. Paramagnetic precharged particles can be used to isolate
polyhistidine- or HQ-tagged protein from 50–500µl of
TNT® Reticulocyte
Lysate with minimal copurification of hemoglobin.
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