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Abstract for siSTRIKE™ U6 Hairpin Cloning System—hMGFP
The siSTRIKE™ U6 Hairpin Cloning System—hMGFP is a
cloning system for short hairpin
RNA (shRNA) expression and includes a vector with an internal fluorescent marker for monitoring delivery
efficiency of shRNA-expressing constructs. The vector contains the human U6 promoter,
which is recognized by RNA polymerase III, allowing transcription of hairpin target
sequences and generation of short interfering RNA (siRNA) in vivo. The CMV promoter
drives the strong constitutive expression of an improved synthetic version of green
fluorescent protein (hMGFP) in many cell types. The presence of the hMGFP gene enables
transfection efficiency to be easily determined and allows selection of transfected
cells by fluorescence-activated cell sorting.
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