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Abstract for Caspase-Glo® 9 Assay
The Caspase-Glo® 9 Assay is a homogeneous luminescent assay that measures
caspase-9 activity. The assay provides a luminogenic caspase-9 substrate in a buffer
system optimized for caspase activity, luciferase activity and cell lysis. The addition
of a single Caspase-Glo® 9 Reagent in an "add-mix-measure" format results in
cell lysis, followed by caspase cleavage of the substrate, and generation of a "glow-type"
luminescent signal (produced by the luciferase reaction). The signal generated is proportional
to the amount of caspase activity present.
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