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Abstract for Caspase-Glo® 8 Assay
The Caspase-Glo® 8 Assay is a homogeneous luminescent assay that measures
caspase-8 activity. The assay provides a luminogenic caspase-8 substrate in a
buffer system optimized for caspase activity, luciferase activity and cell
lysis. The addition of a single Caspase-Glo® 8 Reagent in an "add-mix-measure"
format results in cell lysis, followed by caspase cleavage of the substrate, and generation
of a "glow-type" luminescent signal (produced by the luciferase reaction).
The signal generated is proportional to the amount of caspase activity present.
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