Abstract for Caspase-Glo^® 8 Assay

Part# TB332
Printed in USA. Revised 6/09.
Instructions for Use of Products G7220 & G7351.

The Caspase-Glo^® 8 Assay is a homogeneous luminescent assay that measures caspase-8 activity. The assay provides a luminogenic caspase-8 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo^® 8 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate, and generation of a "glow-type" luminescent signal (produced by the luciferase reaction). The signal generated is proportional to the amount of caspase activity present.