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Abstract for siCHECK™ Vectors
The psiCHECK™-1 Vector and psiCHECK™-2 Vector are
designed to provide a quantitative and rapid approach for
optimization of RNA interference (RNAi). The vectors enable
monitoring of changes in expression of a target gene fused to the
reporter gene. In both vectors, Renilla luciferase is used as
a primary reporter gene, and the gene of interest can be cloned into
the multiple cloning region located downstream of the Renilla
translational stop codon. Initiation of the RNAi process towards a
gene of interest results in cleavage and subsequent degradation of
fusion mRNA. Measurement of decreased Renilla luciferase
activity is a convenient indicator of RNAi effect.
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