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Abstract for MagneSil® Total RNA mini-Isolation System
The MagneSil® Total RNA mini-Isolation System provides a
high-throughput 96-well format for fast, simple preparation of intact,
purified total RNA from small amounts of cell culture (≤1 ×
105 tissue culture cells), tissue (≤2mg tissue lysate
in 100μl), or freshly isolated whole blood (≤20μl). The
protocol enables manual purification with the use of a multichannel
pipettor and plate shaker or high-throughput automated purification on
a variety of liquid-handling workstations. Isolation of total RNA in
a 384-well format from cell culture (≤1 × 103 cells)
and freshly isolated whole blood (≤5μl) may also be performed.
Total RNA purification is achieved without the need for vacuum filtration,
centrifugation or precipitation. The 96-well total RNA isolation procedure
takes about 30 minutes to complete on a liquid-handling workstation.
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