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Abstract for P450-Glo™ Assays

The P450-Glo™ Assays provide a luminescent method for measuring cytochrome P450 activity. A conventional cytochrome P450 reaction is performed by incubating the cytochrome P450 and a luminogenic cytochrome P450 substrate. The substrates of the P450-Glo™ Assays are derivatives of beetle luciferin. The derivatives are not substrates for luciferase but are converted by cytochrome P450s to luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of the cytochrome P450. All of the assays are biochemical assays (i.e., they may be performed using purified components or subcellular fractions). Many of these assays also can be used for cell-based CYP450 induction assays.

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Part# TB325
Printed in USA. Revised 5/07.
Instructions for Use of Products V8751, V8752, V8761, V8762, V8771, V8772, V8781, V8782, V8791, V8792, V8801, V8802, V8811, V8812, V8881, V8882, V8891, V8892, V890, V8902, V8911 & V8912: Request this protocol.

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