Abstract for P450-Glo™ Assays
Part# TB325
Printed in USA. Revised 3/09.
Instructions for Use of Products V8751,
V8752,
V8761,
V8762,
V8771,
V8772,
V8781,
V8782,
V8791,
V8792,
V8801,
V8802,
V8811,
V8812,
V8881,
V8882,
V8891,
V8892,
V8901,
V8902,
V8911,
V8912,
V9001 &
V9002
.
The P450-Glo™ Assays provide a luminescent method to
measure cytochrome P450 activity. A conventional cytochrome
P450 reaction is performed by incubating the cytochrome P450 and
a luminogenic cytochrome P450 substrate. The substrates of the
P450-Glo™ Assays are derivatives of beetle luciferin. The
derivatives are not substrates for luciferase but are converted
by cytochrome P450s to luciferin, which in turn reacts with luciferase
to produce light. The amount of light produced is directly proportional
to the activity of the cytochrome P450. All of the assays are
biochemical assays (i.e., they may be performed using purified
components or subcellular fractions). Many of these assays also can
be used for cell-based CYP450 induction assays.
|
