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Abstract for Caspase-Glo® 3/7 Assay
The Caspase-Glo® 3/7 Assay is a luminescent assay that measures
caspase-3
and -7 activities in purified enzyme preparations
or cultures of adherent or suspension cells. The assay provides a proluminescent
caspase-3/7 substrate, which contains the tetrapeptide sequence DEVD.
This substrate is cleaved to release aminoluciferin, a substrate of
luciferase used in the production of light. The Caspase-Glo® 3/7 Reagent
has been optimized for caspase activity, luciferase activity and cell lysis. The addition of
the single
Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed
by caspase cleavage of the substrate and generation of a “glow-type” luminescent signal. The
Caspase-Glo® 3/7 Assay is designed for use with multiwell plate formats, making it ideal
for automated high-throughput screening of caspase activity or apoptosis.
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