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Abstract for CytoTox-ONE™ Homogeneous Membrane Integrity Assay
The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a
homogeneous, fluorometric method for estimating the number of
non-viable cells present in multiwell plates. Cell viability is most
often defined based on the integrity of the cell membrane and is
most commonly measured by observing the exclusion of Trypan blue or
other vital dyes. Measurement of the leakage of components from the
cytoplasm into the surrounding culture medium has been widely
accepted as a valid method to estimate the number of non-viable
cells.
The CytoTox-ONE™ Assay is a rapid, fluorescent measure of the
release of lactate dehydrogenase (LDH) from cells with a damaged
membrane. LDH released into the culture medium is measured with a
10-minute coupled enzymatic assay that results in the conversion of
resazurin into resorufin. The CytoTox-ONE™ Reagent mix does not
damage healthy cells; therefore, researchers can measure released
LDH directly in assay wells containing a mixed population of viable
and damaged cells.
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