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Abstract for the pCMVTNT™ Vector Technical Bulletin
The pCMVTNT™ Vector is designed for the convenient expression of cloned genes using in vivo or in vitro expression systems. Both the SP6 and the T7 polymerase promoters lie in tandem adjacent to the multiple cloning site, allowing for highly efficient synthesis of RNA in vitro from either promoter. Protein can be expressed in vitro from a gene cloned into the pCMVTNT™ Vector using an SP6- or T7-based, coupled in vitro transcription/translation system. The
pCMVTNT™ Vector contains a 5´ ß-globin leader sequence reported to enhance expression of certain genes in vitro. For in vivo expression, the vector contains a cytomegalovirus
(CMV) enhancer/promoter
region that allows strong constitutive expression in many cell types. A ß-globin/IgG chimeric intron and a late SV40 polyadenylation site are located downstream from the enhancer/promoter region.
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