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Abstract for T7 RiboMAX™ Express Large Scale RNA Production System
The T7 RiboMAX™ Express Large Scale RNA Production System is an in vitro
transcription system designed for the consistent production of milligram amounts
of RNA in a short amount of time. This is about 10- to 20-fold more RNA than is
produced in a standard Riboprobe® System transcription reaction. The
T7 RiboMAX™ Express System reaction differs from those of the Riboprobe®
System in three primary ways: a HEPES (pH 7.5) buffer is used rather than a
Tris-HCl (pH 7.9) buffer, NTP and magnesium concentrations are elevated and
inorganic pyrophosphatase is included in the reaction.
The T7 RiboMAX™ Express
Large Scale RNA Production System differs from the standard RiboMAX™ System in
two primary ways:
• Time savings: The T7 RiboMAX™ Express System produces
milligram amounts of RNA in as little as 30 minutes rather than the 2–4 hours
as required with other commercially available systems including the original
RiboMAX™ System.
• Convenience: The rNTPs and Transcription Buffer have
been combined, thus minimizing pipetting errors and setup time.
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