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Abstract for DNA IQ™ System™—Database Protocol
The DNA IQ™ System is a DNA isolation and quantitation system designed
specifically for the forensic and paternity community. This system employs a
novel technology with magnetic particles to prepare clean samples for short
tandem repeat (STR) analysis easily and efficiently. The DNA IQ™ System
can be used to extract DNA from stains or liquid samples such as blood or resulting
DNA solutions.
The DNA IQ™ resin has a defined DNA-binding capacity in the presence of
excess DNA. Yields will be consistent within a single sample type but will differ
with different sample types. The typical yield for FTA®
blood-card punches is 50–100ng, for liquid blood, 50–200ng; and for
buccal swabs, 100–500ng. Since recovery depends on the sampling method,
solid support and sample type, laboratories will need to determine the average
yield for a single sample type. Once this average yield has been determined, the
researcher can bypass the quantitation step typically necessary with other purification
procedures. |