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Abstract for Apo-ONE® Homogeneous Caspase-3/7 Assay
The Apo-ONE® Homogeneous Caspase-3/7 Assay provides the
necessary reagents for fast and sensitive measurement of the activities of
caspase-3 and -7. These members of the cysteine aspartic acid-specific protease
(caspase) family play key effector roles in apoptosis in mammalian cells. The Apo-ONE® Homogeneous Caspase-3/7 Assay provides a profluorescent
substrate with an optimized bifunctional cell lysis/activity buffer for
caspase-3/7 (DEVDase) activity assays. This assay system may be used with
purified enzyme preparations, cell extracts, or cultures of adherent, suspension
or primary cells. This assay can be flexibly configured for use in
high-throughput systems. The assay is easily adaptable for 96-well and 384-well
formats.
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