Abstract for Wizard^® SV 9600 Plasmid Purification System

Part# TB292
Printed in USA. Revised 5/09.
Instructions for Use of Product A2258.

This system requires the use of the Vac-Man^® 96 Vacuum Manifold or compatible vacuum manifold. Plasmid DNA is purified from bacterial lysates using a 96-well vacuum filtration step to simultaneously clear the bacterial lysate and bind plasmid DNA, eliminating the need for centrifugation. Removal of the Lysate Clearing Plate and subsequent washing of the bound plasmid DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need for emptying waste collection vessels during plasmid DNA recovery. DNA is collected by elution into a 96-well plate.