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Abstract for Wizard® SV 9600 Plasmid Purification System
The Wizard® SV 9600 Plasmid DNA Purification System provides a
simple and reliable method for the rapid isolation of plasmid DNA from 96
samples at one time. The entire miniprep procedure can be completed in 60
minutes or less. The system may be used with automated robotics (e.g., the
Biomek® 2000 Workstation) to yield purified plasmid suitable for
automated fluorescent DNA sequencing as well as for other standard molecular
biology techniques, including restriction enzyme digestion.
This system requires the use of the Vac-Man® 96 Vacuum Manifold
or compatible vacuum manifold. Plasmid DNA is purified from bacterial lysates
using a 96-well vacuum filtration step to simultaneously clear the bacterial lysate and bind plasmid DNA, eliminating the need for centrifugation. Removal of
the Lysate Clearing Plate and subsequent washing of the bound plasmid DNA
requires no disassembly of the manifold, and filtrate waste products are
delivered directly to a vacuum trap, eliminating the need for emptying waste
collection vessels during plasmid DNA recovery. DNA is collected by elution into
a 96-well plate.
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