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Abstract for Wizard® Magnetic 96 DNA Plant System
The Wizard® Magnetic 96 DNA Plant System eliminates lengthy
incubations and the use of hazardous organic solvents such as chloroform and
phenol while gaining the ability to perform plant genomic DNA purification
procedures in a 96-well plate format. These procedures can be performed manually
or automated on a Beckman Coulter Biomek® 2000 workstation. The
Wizard® Magnetic 96 DNA Plant System is designed for purification of
DNA from commercially important agricultural crops such as corn and tomato leaf
as well as canola and sunflower seeds. The DNA purified from these samples can
be used in PCR as well as more stringent applications such as RAPD analysis. The
Wizard® Magnetic 96 DNA Plant System utilizes MagneSil®
Paramagnetic Particles (PMPs). Paramagnetic particles can be considered a
“mobile solid phase”. Unlike column-based systems, the binding of nucleic
acids to magnetic particles can occur in solution, resulting in increased
binding kinetics and binding efficiency.
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