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Abstract for CellTiter-Glo®
Luminescent Cell Viability Assay
The CellTiter-Glo® Luminescent Cell Viability Assay is a
homogeneous method of determining the number of viable cells in culture based on
quantitation of the ATP present, which signals the presence of metabolically
active cells. The CellTiter-Glo®
Assay is designed for use with
multiwell plate formats making it ideal for automated high-throughput screening
(HTS), cell proliferation and cytotoxicity assays. The homogeneous assay
procedure involves addition of a single reagent (CellTiter-Glo®
Reagent) directly to cells cultured in serum-supplemented medium. Cell washing,
removal of medium or multiple pipetting steps are not required.
The homogeneous "add-mix-measure" format results in cell lysis and
generation of a luminescent signal proportional to the amount of ATP present .
The amount of ATP is directly proportional to the number of cells present in
culture in agreement with previous reports. The CellTiter-Glo®
Assay
generates a "glow-type" luminescent signal, produced by the luciferase
reaction, which has a half-life of greater than five hours. This extended
half-life eliminates the need for reagent injectors and provides flexibility for
continuous or batch-mode processing of multiple plates. The unique homogeneous
format reduces pipetting errors that may be introduced during the multiple steps
required by other ATP measurement methods.
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