Abstract for Wizard® MagneSil® Sequencing Reaction Clean-Up System
Part# TB287
Printed in USA. Revised 6/09.
Instructions for Use of Products A1830,
A1831,
A1832,
A1835 &
A8231.
The MagneSil® Paramagnetic Particles allow purification of nucleic
acids such as plasmid DNA and PCR fragments. This technology also can be applied to
the high-throughput purification of BigDye® terminator DNA sequencing
reactions prior to automated sequence analysis. Procedures exist for several robotic
workstations using the standard 96-well amplification plate format, offering the
distinct advantage of a hands-off protocol. Sequence quality is similar to or better
than standard manual methods. No user intervention is required from the time the
96-well amplification plate is put on the robot deck until the samples are loaded
onto the DNA sequencer.
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