Abstract for SignaTECT® Calcium/Calmodulin-Dependent Protein Kinase
(CaM KII) Assay System
The SignaTECT® Calcium/Calmodulin-Dependent Protein Kinase (CaM
KII) Assay System provides an improved method by which to quantitate CaM KII
protein kinase activity, both in purified enzyme preparations and in cell or
tissue extracts. The SignaTECT® CaM KII System utilizes the unique
SAM2® Biotin Capture Membrane, produced by a proprietary process
that results in a high density of streptavidin on the membrane matrix.
The SignaTECT® CaM KII Assay System overcomes the problem of
nonspecific substrate binding by using a biotinylated peptide that is selective
for CaM KII in conjunction with the SAM2® Membrane. The high binding
capacity of the SAM2® Membrane for the CaM KII biotinylated peptide
substrate and the low backgrounds observed with this system maximize the
signal-to-noise ratio.
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