Abstract for SignaTECT® Calcium/Calmodulin-Dependent Protein Kinase
(CaM KII) Assay System
Part# TB279
Printed in USA. Revised 6/09.
Instructions for Use of Product V8161.
The SignaTECT® Calcium/Calmodulin-Dependent Protein Kinase
(CaM KII) Assay System provides an improved method to quantitate CaM KII protein
kinase activity in both purified enzyme preparations and cell or tissue extracts.
The SignaTECT® CaM KII System uses the unique SAM2®
Biotin Capture Membrane, produced by a proprietary process that results in a high
density of streptavidin on the membrane matrix.
The SignaTECT® CaM KII Assay System overcomes the problem of
nonspecific substrate binding by using a biotinylated peptide that is selective
for CaM KII in conjunction with the SAM2® Membrane. The high binding
capacity of the SAM2® Membrane for the CaM KII biotinylated peptide
substrate and low backgrounds observed with this system maximize the signal-to-noise ratio.
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