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Abstract for Wizard® SV 96 Plasmid DNA Purification System
The Wizard® SV 96 Plasmid DNA Purification System provides a
simple and reliable method for the rapid isolation of plasmid DNA from as many
as 96 samples. The entire miniprep procedure can be completed in 60 minutes or
less. The purified plasmid can be used directly for automated fluorescent DNA
sequencing as well as other standard molecular biology techniques, including
restriction enzyme digestion.
Plasmid DNA is purified from bacterial lysates using a 96-well vacuum
filtration step to simultaneously clear the bacterial lysate and bind plasmid
DNA, eliminating the need for centrifugation. Washing of the bound plasmid DNA
requires removal of only the Lysate Clearing
Plate and Manifold Collar. Filtrate waste products are delivered directly to a vacuum
trap, eliminating the need for emptying waste collection flasks during DNA
recovery. DNA is collected by eluting into a 96-well plate.
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