Abstract for Anti-ACTIVE^® CaM KII pAb, (pT²⁸⁶) and Anti-ACTIVE^® Qualified Secondary Antibody Conjugates

Part# TB264
Printed in USA. Revised 6/09.
Instructions for Use of Products V1111, V7951 & V7971.

Promega has developed a polyclonal antibody (pAb) to the multifunctional calcium/calmodulin-dependent protein kinase CaM kinase II (CaM KII) that is phosphorylated on threonine 286 (pT²⁸⁶). The Anti-ACTIVE^® CaM KII pAb was raised against the phosphothreonine-containing peptide. The recommended dilution of Anti-ACTIVE^® CaM KII pAb for Western blot analysis is 1:5,000.

Donkey Anti-Rabbit IgG, (H + L), HRP and Donkey Anti-Rabbit IgG, (H + L), AP are affinity purified-horseradish peroxidase-(HRP) or alkaline phosphatase-(AP) conjugated secondary antibodies for use with Anti-ACTIVE^® pAbs in Western blot analysis using chemiluminescent and colorimetric detection methods. The antibody conjugates exhibit minimal cross-reactivity to goat, mouse and sheep IgG, bovine serum albumin (BSA) and proteins in mammalian cell extracts. These antibody conjugates provide low backgrounds and highly specific signals when used at the recommended 1:10,000 dilution with Anti-ACTIVE^® CaM KII pAb.