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Abstract for TaqBead™ Hot Start Polymerase
Promega’s TaqBead™ Hot Start Polymerase facilitates
hot-start PCR by keeping the enzyme sequestered in paraffin wax until the
reaction temperature reaches approximately 60°C. This technique increases PCR
specificity by keeping the polymerase separate from the rest of the reaction
components until a critical temperature is reached, thus decreasing the
probability of amplifying products that are the result of nonspecific binding of
primers to each other or to template DNA.
Hot-start PCR protocols increase amplification specificity and amplimer yield
by creating conditions that minimize the possibility of nonspecific priming,
primer-dimer formation or other reactions, which can occur at low temperatures
once all the PCR reaction components are mixed.
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