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Abstract for CellTiter 96® AQueous One Solution Cell
Proliferation Assay
The CellTiter 96® AQueous One Solution Cell
Proliferation Assay is a colorimetric method for determining the number of
viable cells in proliferation or cytotoxicity assays. The CellTiter 96®
AQueous One Solution Reagent contains a novel tetrazolium compound
[3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium,
inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES).
PES has enhanced chemical stability, which allows it to be combined with MTS to
form a stable solution. This convenient "One Solution" format is an
improvement over the first version of the CellTiter 96® AQueous
Assay, where phenazine methosulfate (PMS) is used as the electron coupling
reagent, and PMS Solution and MTS Solution are supplied separately.
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