Abstract for CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay

Part# TB245
Printed in USA. Revised 6/09.
Instructions for Use of Products G3580, G3581 & G3582.

The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay is a colorimetric method to determine the number of viable cells in proliferation or cytotoxicity assays. The CellTiter 96^® AQ_ueous One Solution Reagent contains a novel tetrazolium compound [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution. This convenient "One Solution" format is an improvement over the first version of the CellTiter 96^® AQ_ueous Assay, where phenazine methosulfate (PMS) is used as the electron coupling reagent, and PMS Solution and MTS Solution are supplied separately.