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Abstract for CellTiter 96® AQueous One Solution Cell Proliferation Assay

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The CellTiter 96® AQueous One Solution Reagent contains a novel tetrazolium compound [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution. This convenient "One Solution" format is an improvement over the first version of the CellTiter 96® AQueous Assay, where phenazine methosulfate (PMS) is used as the electron coupling reagent, and PMS Solution and MTS Solution are supplied separately.


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Part# TB245
Printed in USA. Revised 12/07.
Instructions for Use of Products G3580, G3581 & G3582: Request this protocol.

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