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Abstract for NT-3 E_max^® ImmunoAssay System

The NT-3 E_max^® ImmunoAssay System is designed for the sensitive and specific detection of Neurotrophic Factor-3 (NT-3) in an antibody sandwich format. In this format, flat-bottom 96-well plates are coated with Anti-Human NT-3 pAb that binds soluble NT-3. A quick wash removes any unbound NT-3. Captured NT-3 is bound by a second, specific antibody, Anti-NT-3 mAb. After washing, a tertiary antibody conjugated to horseradish peroxidase (HRP), Anti-Mouse IgG, HRP Conjugate, is added and binds the sandwich complex. Finally, the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) is added. The amount of specifically bound NT-3 is proportional to the color generated in the coupled oxidation-reduction reaction and is quantitated against a standard curve generated with known amounts of NT-3. 

The NT-3 E_max^® ImmunoAssay System offers several benefits:

• Specificity: Specific detection of NT-3; typically less than 3% cross-reactivity to other related neurotrophic factors at 10ng/ml.
• Sensitivity: Detects a minimum of 10pg/ml of NT-3.
• Flexibility: Available in sizes for five or two 96-well ELISA plates; can configure plates as desired.
• High Value: Optimized reagents and protocol.

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Part# TB243
Printed in USA. Revised 9/05
Instructions for Use of Products G7640 & G7641: Request this protocol.

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