Abstract for NT-3 Emax® ImmunoAssay System
Part# TB243
Printed in USA. Revised 6/09.
Instructions for Use of Products G7640
& G7641.
The NT-3 Emax® ImmunoAssay System is designed for
the sensitive and specific detection of neurotrophic factor-3 (NT-3) in an antibody
sandwich format. In this format, flat-bottom 96-well plates are coated with
Anti-Human NT-3 pAb, which binds soluble NT-3. A quick wash removes any unbound NT-3.
Captured NT-3 is bound by a second, specific antibody, Anti-NT-3 mAb. After washing,
a tertiary antibody conjugated to horseradish peroxidase (HRP), Anti-Mouse IgG, HRP
Conjugate, is added and binds the sandwich complex. Finally, the chromogenic substrate
3,3′,5,5′-tetramethylbenzidine (TMB) is added. The amount of specifically
bound NT-3 is proportional to the color generated in the coupled oxidation-reduction
reaction and is quantitated using a standard curve generated with known amounts of NT-3.
The NT-3 Emax® ImmunoAssay System offers several benefits:
- Specificity: Specifically detects NT-3; typically less than 3% cross-reactivity
to other related neurotrophic factors at 10ng/ml.
- Sensitivity: Detects a minimum of 10pg/ml of NT-3.
- Flexibility: Available in sizes for five or two 96-well ELISA plates; can
configure plates as desired.
- High Value: Provides optimized reagents and protocol.
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