Abstract for SignaTECT^® Protein Kinase C (PKC) Assay System

Part# TB242
Printed in USA. Revised 6/09.
Instructions for Use of Product V7470.

The SignaTECT^® Protein Kinase C (PKC) Assay System uses the biotinylated peptide Neurogranin _(28–43) (AAKIQAS*FRGHMARKK), a specific substrate commercially available for PKC activity. The biotinylated, ³²P-labeled substrate is recovered from the reaction mix using the SAM^2® Biotin Capture Membrane, which is a novel streptavidin matrix. The SAM^2® Membrane is prenumbered and partially cut so that individual squares can be easily identified, separated and placed in scintillation vials. Alternatively, the intact SAM^2® Membrane can be analyzed by phosphorimaging or conventional autoradiography. This convenient and rapid technique provides low backgrounds and high signal-to-noise ratios even with complex samples such as crude cell extracts, while retaining the high substrate capacity necessary to maintain optimum reaction kinetics.