|
Download
protocol
265kb
pdf? |
|
Abstract for pRL-SV40 Vector
The pRL-SV40 Vector is intended for use as an internal control reporter and
may be used in combination with any experimental reporter vector to co-transfect
mammalian cells. All of the pRL Reporter Vectors contain a cDNA (Rluc)
encoding Renilla luciferase, which was originally cloned from the
marine organism Renilla reniformis (sea pansy). As described in TB239,
the Renilla luciferase cDNA contained within the pRL Vectors has been
modified slightly to provide greater utility.
The pRL-SV40 Vector contains the SV40 enhancer and early promoter elements to
provide high-level expression of Renilla luciferase in cotransfected
mammalian cells. Renilla luciferase is a 36kDa monomeric protein that
does not require post-translational modification for activity. Therefore, like
firefly luciferase, the enzyme may function as a genetic reporter immediately
following translation. |
