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Abstract for pRL-null Vector
The pRL-null Vector is intended for use in constructing a control reporter
vector that may be used in combination with any experimental reporter vector to
co-transfect mammalian cells. All of the pRL Reporter Vectors contain a
cDNA (Rluc) encoding Renilla luciferase, which was originally
cloned from the marine organism Renilla reniformis (sea pansy). As
described in the Technical Bulletin, the Renilla luciferase cDNA
contained within the pRL Vectors has been modified slightly to provide greater
utility.
The pRL-null Vector contains no enhancer or promoter elements. Rather, it
contains a multiple cloning region upstream of Rluc to allow for the
cloning of any desired regulatory element(s) to drive expression of Renilla
luciferase. Renilla luciferase is a 36kDa monomeric protein that does
not require post-translational modification for activity. Therefore, like
firefly luciferase, the enzyme may function as a genetic reporter immediately
following translation.
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