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Abstract for DeadEnd™ Fluorimetric TUNEL System
The DeadEnd™ Fluorimetric TUNEL System is designed for the specific
detection and quantitation of apoptotic cells within a cell population. The
system is non-radioactive and provides for simple, accurate and rapid detection
of apoptotic cells in situ at the single cell level or in cell suspensions. The
system can be used to assay apoptotic cell death in a wide variety of systems,
including cultured cells and formalin-fixed, paraffin-embedded tissue sections.
The DeadEnd™ Fluorimetric TUNEL System measures nuclear DNA fragmentation, an
important biochemical hallmark of apoptosis in many cell types.
Assay Principle: The DeadEnd™ Fluorimetric TUNEL System measures the fragmented DNA of
apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3´-OH DNA
ends using the Terminal Deoxynucleotidyl Transferase, Recombinant, enzyme (rTdT), which forms a
polymeric tail using the principle of the TUNEL (TdT-mediated dUTP
Nick-End Labeling) assay. The fluorescein-12-dUTP-labeled
DNA can then either be visualized directly by fluorescence microscopy or
quantitated by flow cytometry.
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