Abstract for DeadEnd™ Fluorometric TUNEL System

Part# TB235
Printed in USA. Revised 7/09.
Instructions for Use of Product G3250.

The DeadEnd™ Fluorometric TUNEL System measures the fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-12-dUTP at 3´-OH DNA ends using the Terminal Deoxynucleotidyl Transferase, Recombinant, enzyme (rTdT), which forms a polymeric tail using the principle of the TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. The fluorescein-12-dUTP-labeled DNA can then either be visualized directly by fluorescence microscopy or quantitated by flow cytometry.