Abstract for Griess Reagent System
Part# TB229
Printed in USA. Revised 6/09.
Instructions for Use of Product G2930.
The Griess Reagent System is based on the chemical diazotization reaction that
was originally described by Griess in 1879, which uses sulfanilamide and
N-1-napthylethylenediamine dihydrochloride (NED) under acidic (phosphoric
acid) conditions. This system detects NO2– in a
variety of biological and experimental liquid matrices such as plasma, serum,
urine and tissue culture medium. The nitrite sensitivity depends on the matrix.
The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized
distilled water).
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