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Abstract for Griess Reagent System
The Griess Reagent System is based on the chemical diazotization
reaction that was originally described by Griess in 1879, which uses
sulfanilamide and N-1-napthylethylenediamine dihydrochloride (NED)
under acidic (phosphoric acid) conditions. This system detects NO2–
in a variety of biological and experimental liquid matrices such as plasma,
serum, urine and tissue culture medium. The nitrite sensitivity is dependent on
the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure,
deionized distilled water).
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