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Abstract for AgarACE™ Agarose-Digesting Enzyme
AgarACE™ Enzyme is a unique agarose-digesting enzyme
developed by Promega for simple and quantitative recovery of intact DNA or RNA
from agarose gels. Isolated from a marine Flavobacterium, AgarACE™ Enzyme completely digests agarose to ethanol-soluble oligosaccharides in 15
minutes. AgarACE™ Enzyme is sufficiently thermostable that
low melting point (LMP) agarose melted at 65–75°C does not have to be
equilibrated to the reaction temperature before hydrolysis. Simply place the
tube containing the molten agarose at 42–47°C and add AgarACE™ Enzyme. With slight modifications to the protocol, AgarACE™ Enzyme can also effectively hydrolyze non-LMP agaroses, although the higher
temperatures required to melt them may be deleterious to the DNA or RNA.
Finally, AgarACE™ Enzyme does not require buffer exchange
in order to exhibit high agarose hydrolytic activity; it works equally well in
TAE or TBE buffers used in normal electrophoretic procedures. These advantages
have allowed Promega to design protocols that are considerably faster and easier
to use than other current protocols.
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