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Abstract for NGF E_max^® ImmunoAssay System

The NGF (Nerve Growth Factor) E_max^® ImmunoAssay System is designed for the sensitive and specific detection of NGF in an antibody sandwich format. In this format, flat-bottom 96-well plates are coated with Anti-NGF Polyclonal Antibody (pAb) which binds soluble NGF. The captured NGF is bound by a second specific monoclonal antibody (mAb). After washing, the amount of specifically bound mAb is detected using a species-specific antibody conjugated to horseradish peroxidase (HRP) as a tertiary reactant. The unbound conjugate is removed by washing, and following an incubation with a chromogenic substrate, the color change is measured. The amount of NGF in the test solutions is proportional to the color generated in the oxidation-reduction reaction. Using this system, NGF in tissue culture supernatants or tissue extracts can be quantitated.

The NGF E_max^® ImmunoAssay System offers several benefits:

• Specificity: Specific detection of NGF; typically less than 3% cross-reactivity with other neurotrophic factors at 10ng/ml.
• Sensitivity: Detects a minimum of 7.8pg/ml of NGF.
• Flexibility: Available in sizes for two or five 96-well ELISA plates; can configure plates as desired.
• High Value: Optimized reagents and protocol.

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Part# TB226
Printed in USA. Revised 3/07
Instructions for Use of Products G7630 & G7631: Request this protocol.

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