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Abstract for Wizard® Plus SV Minipreps DNA
Purification System
The Wizard® Plus SV Minipreps DNA
Purification System provides a simple and reliable method for rapid
isolation of plasmid DNA. The entire miniprep procedure can be
completed in 30 minutes or less, depending on the number of samples
processed. This system can be used to isolate any plasmid from E.
coli hosts but works
most efficiently when the plasmid is less than 20,000bp in size. Purified plasmids can be used without further manipulation and also can be used for in
vitro transcription reactions when supplemented with a ribonuclease
inhibitor such as Recombinant RNasin® Ribonuclease
Inhibitor (Cat.# N2511).
The protocol presented in this Technical Bulletin is for the
isolation of plasmid DNA from E. coli. Plasmid DNA can be
purified from 1–10ml of overnight cultures of E. coli with
the Wizard® Plus SV Minipreps DNA Purification
System. The yield of plasmid will vary depending on a number of
factors, including the volume of bacterial culture, plasmid copy
number, type of culture medium and the bacterial strain used.
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