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Abstract for TGFβ₂ E_max^® ImmunoAssay System

The TGFβ₂ E_max^® ImmunoAssay System is designed for the sensitive and specific detection of biologically active Transforming Growth Factor β₂ (TGFβ₂) in an antibody sandwich format. In this format, flat-bottom 96 well plates are coated with TGFβ Coat mAb, which binds soluble TGFβ₂. A second antibody, Anti-TGFβ₂ pAb, is added to complete the sandwich. After washing, antibody conjugate (horseradish peroxidase, TGFβ HRP) is added and binds the sandwich complex. Finally, the chromogenic substrate 3,3´,5,5´-tetramethyl benzidine (TMB) is added. The amount of specifically bound TGFβ₂ in the sample is proportional to the color generated in the coupled oxidation-reduction reaction and is quantitated against a standard curve generated with known amounts of TGFβ₂. Using this system, biologically active TGFβ₂ in media, plasma, serum or urine can be quantitated in the range of 32-1,000pg/ml.

The TGFβ₂ E_max^® ImmunoAssay System offers several benefits:

• Specificity: Specific detection of TGFβ₂; <=3% cross-reactivity with TGFβ₁ and TGFβ₃ at 10ng/ml.
• Sensitivity: Detects a minimum of 32pg/ml of TGFβ₂.
• Flexibility: ELISA plates can be set up in any desired configuration.
• High Value: Optimized reagents and protocol.

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Part# TB224
Printed in USA. Revised 2/01
Instructions for Use of Product G7600: Request this protocol.

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