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Abstract for Access RT-PCR System
The Access RT-PCR System is designed for the reverse
transcription (RT) and polymerase chain reaction (PCR) amplification of a
specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme
system provides sensitive, quick, and reproducible analysis of even rare RNAs.
The system uses AMV Reverse Transcriptase (AMV RT) from Avian Myeloblastosis
Virus for first strand DNA synthesis and the thermostable Tfl DNA
Polymerase from Thermus flavus for second strand cDNA synthesis and DNA
amplification. The Access RT-PCR System includes an optimized single-buffer
system that permits extremely sensitive detection of RNA transcripts without a
requirement for buffer additions between the reverse transcription and PCR
amplification steps. This simplifies the procedure and reduces the potential for
contaminating the samples. In addition, the improved performance of AMV Reverse
Transcriptase at elevated temperatures (45°C) in the AMV/Tfl 5X Reaction
Buffer minimizes problems encountered with secondary structures in RNA.
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