Abstract for E. coli T7 S30 Extract System for Circular DNA

Part# TB219
Printed in USA. Revised 3/09.
Instructions for Use of Products G7220 & G7351.

The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or lambda vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all necessary components for translation. The investigator supplies only cloned DNA containing a T7 promoter and a ribosome binding site. The T7 S30 Extract for Circular DNA Templates is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and lon protease activity. This results in greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo.