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Abstract for E. coli T7 S30 Extract System for Circular DNA
The E. coli T7 S30 Extract System for Circular DNA simplifies the
transcription/translation of DNA sequences cloned in plasmid or lambda vectors
containing a T7 promoter by providing an extract that contains T7 RNA polymerase
for transcription and all necessary components for translation. The investigator
supplies only cloned DNA containing a T7 promoter and a ribosome binding site.
The T7 S30 Extract for Circular DNA Templates is prepared by
modifications of the method described by Zubay from an E. coli strain B
deficient in OmpT endoproteinase and lon protease activity. This results in
greater stability of expressed proteins that would otherwise be degraded by
proteases if expressed in vivo. |
