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Abstract for Tfx™-20 and Tfx™-50 Reagents for the Transfection
of Eukaryotic Cells
The ability to introduce DNA into eukaryotic cells provides a powerful means
by which to study the function and control of mammalian genes. Common techniques
for gene (DNA) transfer into eukaryotic cells include calcium phosphate,
DEAE-Dextran, electroporation and more recently, liposome-mediated
transfection.
The Tfx™ Reagents are a mixture of L-dioleoyl phosphatidylethanolamine (DOPE)
and a synthetic, cationic lipid molecule [N,N,N´,N´-tetramethyl-N,N´-bis(2-hydroxyethyl)-2,3-di(oleoyloxy)-1,4-butanediammonium
iodide]. All Tfx™ Reagents contain the same concentration of the cationic
lipid component but are formulated with different molar ratios of the fusogenic
lipid, DOPE. The Tfx™ Reagents are supplied as dried lipid films. Upon
hydration with water, these lipids form multilamellar vesicles that associate
with nucleic acids and presumably facilitate their transfer into cells by fusion
of the vesicles with the cell membrane. The Tfx™ Reagents may be used to
transfect cells in the presence of serum. This can be advantageous for
transfecting primary cells, which tend to lose viability without serum. The
optimal transfection conditions for a specific cell type must be determined
experimentally.
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