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Abstract for SignaTECT^® Protein Tyrosine Kinase Assay System

Protein tyrosine kinases (PTKs) play a critical role in the modulation of many cellular events, including differentiation, growth and metabolism. Phosphorylation of tyrosine residues is essential for maintaining cellular homeostasis, yet this post-translational modification also provides the means by which a number of cellular oncogenes deregulate various signaling pathways and induce transformation. This class of enzymes is therefore an important target for both basic research and drug development.

PTKs represent a diverse group of protein kinases, comprising both transmembrane and cytoplasmic enzymes. Although protein phosphorylation is a common modification (approximately 10% of all proteins are phosphorylated), tyrosine phosphorylations make up only 0.01% of these events. Widespread appreciation of the complexity of signaling pathways involving this diverse class of enzymes has resulted in the need for more sophisticated reagents to dissect the role of PTKs in normal and disease states. To meet this need, Promega has developed a novel, broad-specificity SignaTECT^® Protein Tyrosine Kinase Assay System to measure rapidly and specifically the enzymatic activities of a variety of PTKs including both transmembrane receptor PTK enzymes (epidermal growth factor receptor, insulin receptor, platelet-derived growth factor receptor and fibroblast growth factor receptor) and cytosolic PTK enzymes (p60^scr, p93^fes and p43^abl).

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Part# TB211
Printed in USA. Revised 12/06.
Instructions for Use of Product V6480: Request this protocol.

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