|
Download
protocol
207kb
pdf? |
|
Abstract for pSP-luc+NF Fusion Vector
The pSP-luc+NF Fusion Vector is a luciferase cassette vector
containing the engineered firefly luciferase gene, luc+NF. The luc+NF
gene is related to the luc+ gene found in our pGL3 family of
eukaryotic reporter vectors but has been further modified for maximum
flexibility in constructing
N-terminal fusions (NF) with luciferase. The pSP-luc+NF
Fusion Vector is not itself intended for the expression of luciferase in
eukaryotic cells because it does not contain eukaryotic promoters, enhancers or
polyadenylation signals.
The luc+NF gene is positioned downstream of an SP6 promoter and a
ribosome binding site. An opposing T7 promoter is located immediately downstream
of luc+NF. Thus, the pSP-luc+NF Fusion Vector provides a
convenient template for the in vitro synthesis of both sense and anti-sense
luciferase transcripts for studies involving in situ hybridization, RNA
processing, RNA transfection or coupled in vitro transcription/
translation and
protein folding. Multiple cloning regions containing recognition sequences for
commonly used restriction enzymes are positioned at the 5´ and 3´ ends of luc+NF
to provide maximum flexibility in cloning. Subcloning luc+NF into
expression vectors provides a useful genetic reporter with exceptional
sensitivity. Luciferase enzymatic activity can be assayed most efficiently using
the Luciferase Assay System.
The GenBank®/EMBL Accession number for the pSP-luc+NF
Fusion Vector is U47123.
|
