Abstract for pAdVAntage™ Vector
Co-transfection of mammalian cells with the pAdVAntage™ Vector enhances
transient protein expression in a variety of cell types by increasing
translation initiation.
Transfection of mammalian cells with an expression vector often results in
suboptimal expression of the protein of interest. Double-stranded RNA (dsRNA)
generated during transfection is thought to activate the dsRNA-activated
inhibitor (DAI), one of several enzymes involved in the host cell’s antiviral
defense system. DAI phosphorylates the translation initiation factor eIF-2,
halting translation and therefore protein production.
However, inhibition of translation by DAI can be overcome with the adenoviral
Virus Associated I RNA (VAI RNA) that is produced by RNA polymerase III
following co-transfection with the pAdVAntage™ Vector. The VAI RNA binds to
DAI, preventing its activation, thereby allowing translation and protein
expression.
The pAdVAntage™ Vector contains base pairs 9,831-11,555 of the adenovirus
type 2 genome on a 1,724bp SalI-HindIII fragment that
encodes the virus-associated RNA genes, VAI and VAII. |
