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Abstract for DeadEnd™ Colorimetric TUNEL System
The DeadEnd™ Colorimetric TUNEL System is a
non-radioactive system designed to provide simple, accurate and rapid detection
of apoptotic cells in situ at the single cell level. The system can be used to
assay apoptotic cell death in both tissue sections and cultured cells. The
DeadEnd™ Colorimetric TUNEL System measures nuclear
DNA fragmentation, an important biochemical indicator of apoptosis in many cell
types.
Assay Principle: The DeadEnd™ Colorimetric TUNEL System end-labels the fragmented DNA of apoptotic cells using a
modified TUNEL (TdT-mediated dUTP Nick-End Labeling)
assay. Biotinylated nucleotide is incorporated at the 3′-OH DNA ends using the
enzyme Terminal deoxynucleotidyl Transferase, Recombinant (rTdT). Horseradish-peroxidase-labeled
streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides,
which are detected using the peroxidase substrate, hydrogen peroxide, and the
stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei
are stained dark brown.
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