Abstract for TGFβ1 Emax®
ImmunoAssay System
Part# TB196
Printed in USA. Revised 6/09.
Instructions for Use of Products G7590
& G7591.
The TGFβ1 Emax®
ImmunoAssay System is designed for the sensitive and specific detection of
biologically active TGFβ1 in an antibody
sandwich format. In this format, flat-bottom 96 well plates are coated with
TGFβ Coat mAb, which binds soluble TGFβ1.
The captured TGFβ1 is bound by a second
specific polyclonal antibody (pAb). After washing, the amount of specifically
bound pAb is detected using a species-specific antibody conjugated to
horseradish peroxidase (TGFβ HRP Conjugate) as a
tertiary reactant. The unbound conjugate is removed by washing and, following an
incubation with a chromogenic substrate, the color change is measured. The
amount of TGFβ1 in the test solutions is
proportional to the color generated in the oxidation-reduction reaction. Using
this system, biologically active TGFβ1 in
tissue culture supernatants, plasma, serum or urine can be quantitated in the
range of 32-1,000pg/ml.
The TGFβ1 Emax®
ImmunoAssay System offers several benefits:
- Specificity: Specific detection of TGFβ1;
typically less than or equal to 3% cross-reactivity with TGFβ2
and TGFβ3 at 10ng/ml.
- Sensitivity: Detects a minimum of 32pg/ml of TGFβ1.
- Flexibility: ELISA plates can be set up in any desired
configuration.
- High Value: Optimized reagents and protocol.
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