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Abstract for the EGGstract® IgY Purification System
The EGGstract® IgY Purification System provides a quick and easy
method for the isolation of egg yolk immunoglobulin (IgY), the ~180kDa chicken
IgG homolog, from chicken egg yolks. The yolk of eggs laid by immunized chickens
has been recognized as an excellent source of polyclonal antibodies (pAb).
Because a single egg contains as much antibody as an average bleed from a
rabbit, this simple, noninvasive approach represents an appealing alternative to
the generation of pAb in rabbits. Additionally, IgY can be produced to
antigens that fail to elicit a humoral immune response in rabbits or other
mammals due to species relatedness.
Several methods have been described for the purification of IgY from egg
yolks; however, these protocols consist of numerous time-consuming steps,
including salting in, chromatography and dialysis. In contrast, the EGGstract®
IgY Purification System is a rapid and highly effective IgY purification method.
The entire purification procedure can be performed in one hour, with roughly 30
minutes of hands-on time. Using this system, yields typically range between
40–80mg of approximately 75% pure IgY per egg. An optional second precipitation
with Precipitation Solution B can increase IgY purity to approximately 90%. IgY
purified using the EGGstract® System is suitable for immunoblotting,
ELISA, immunostaining and other standard immunological procedures. EGGstract®
System-purified IgY may be used in conjunction with Immobilized Anti-Chicken IgY
for immunoprecipitations and affinity purification.
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