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Abstract for the Transcend™ Non-Radioactive Translation Detection Systems

The Transcend™ Non-Radioactive Translation Detection Systems allow non-radioactive detection of proteins synthesized in vitro. Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [³⁵S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged, ε-labeled biotinylated lysine-tRNA complex (Transcend™ tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection. Typically, 0.5–5ng of protein can be detected by these methods within 3–4 hours after gel electrophoresis.

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Part# TB182
Printed in USA. Revised 6/06
Instructions for Use of Products L5061, L5070 & L5080: Request this protocol.

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