Abstract for Wizard® Plus Midipreps DNA Purification System
Part# TB173
Printed in USA. Revised 4/09.
Instructions for Use of Products A7640,
A7651 &
A7701.
The Wizard® Plus Midipreps DNA Purification System
eliminates many of the problems associated with standard midiprep procedures,
and provides a simple and reliable method for rapid isolation of plasmid DNA.
This system can be used to isolate any plasmid, but works most efficiently with
plasmids <20,000bp. When using the standard protocol, the entire midiprep
process can be completed in 90 minutes or less, with no organic extractions or
ethanol precipitations. Multiple midipreps can be easily processed at one time
with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man®
Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted
from the Wizard® Midicolumn in Nuclease-Free Water (Cat.# P1193).
The purified plasmid can be used directly for automated fluorescent DNA
sequencing or restriction digestion without further manipulation, and also can
be used for in vitro transcription reactions supplemented with a ribonuclease
inhibitor, such as Recombinant RNasin®
Ribonuclease Inhibitor (Cat.# N2511).
|
