Abstract for the TNT^® Coupled Wheat Germ Extract Systems

Part# TB165
Printed in USA. Revised 3/09.
Instructions for Use of Products L4120, L4130, L4140, L5030 & L5040.

The TNT^® Coupled Wheat Germ Extract Systems offers an alternative for eukaryotic in vitro translation: a one-tube, coupled transcription/translation system. The TNT^® Extract Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TNT^® Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT^® Extract reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates.