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Abstract for Wizard® DNA Clean-Up System
Concentration of DNA samples, removal of small nucleotides from reactions and
purification of DNA from proteins or salts all require organic extractions and
precipitation, which can result in low and variable recoveries. The concern over
recovery increases when working with amounts of DNA in the 50–500ng
range. The Wizard® DNA Clean-Up System provides a simple and effective way
to purify linear and circular DNA (200–50,000bp) from the following:
- restriction enzymes, including heat stable restriction enzymes
- phosphatases and kinases
- DNA polymerases, including Taq DNA Polymerase
- exonucleases and endonucleases, including DNase I but not RNases
- mononucleotides
- salts
The entire procedure can be completed in 15 minutes or less, with no organic
extractions or ethanol precipitation. DNA is eluted from the Wizard®
Clean-Up Resin in water or TE buffer, ready for use.
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