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Abstract for Wizard® Plus Megapreps DNA Purification System
Large-scale plasmid preparations, such as cesium chloride purification, can
be both laborious and time-consuming, often requiring an overnight
centrifugation. The Wizard® Plus Megapreps DNA
Purification System is simple and rapid and requires only a centrifuge, a
vacuum source and a vacuum manifold such as the Vac-Man®
(20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample
capacity, Cat.# A7660) Laboratory Vacuum Manifold. The system yields up to one
milligram of high-copy-number plasmid DNA (200–20,000bp) from a 1,000ml culture
in less than three hours.
The Wizard® Plus Megapreps System does not require
organic extractions or cesium chloride gradients. The purified DNA is eluted in
Nuclease-Free Water (Cat.# P1193) or TE buffer. The purified plasmid can be used
directly for DNA sequencing and restriction digestion without further
manipulation and can also be used for in vitro transcription reactions
supplemented with a ribonuclease inhibitor such as Recombinant RNasin®
Ribonuclease Inhibitor (Cat.# N2511).
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